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All functions

AddInfo()
Add information to STID object
AddMetaColumn()
Add columns to existing metadata
AddMetaData()
Add metadata to STID object
AddMSNicheCells()
Add columns to MultiSampNiche cells
AddSSNicheCells()
Add columns to SingleSampNiche cells
AddSSNicheGenes()
Add annotations to SingleSampNiche genes
anno_SingleR()
Annotate Cell Types Using SingleR
as.Seurat(<STID>)
Convert STID object to Seurat object
as.STID()
Convert Seurat object to STID object
CalSampCAI()
Calculate Niche Aggregation Index
CalSampCellComm()
Calculate Niche Cell Communication using CellChat
CalSampCoLoc()
Calculate Niche Co-localization
CalSampComp()
Calculate Niche Cell Type Composition
CalSampDEGs()
Calculate Niche Differential Expression Genes
CalSampGeneCor()
Calculate Niche Gene Expression Correlations
CalSampGeneTrend()
Calculate gene expression trends across time points
CalSampGRN()
Calculate Niche Gene Regulatory Networks using NicheNet
CalSampOSE()
Calculate Spatial Organization Entropy (OSE) for samples
CalSampPathoTrack()
Calculate pathogen tracking across samples
CalSampPPI()
Predict Niche Protein-Protein Interactions
clog() clog_start() clog_end() clog_check() clog_SS() clog_MS() clog_step() clog_loop() clog_normal() clog_title() clog_warn() clog_error()
Logging functions for pipeline execution
COLOR_DIS_CON COLOR_LIST
Predefined color palettes
CompareNiche()
Compare two niche detection results
configure_conda()
Configure Python Conda Environment
CorrectBackground()
Correct background expression in spatial transcriptomics data
CreateMultiSampNiche() CreateMSNiche()
Create a MultiSampNiche object for cross-sample comparison
CreateSingleSampNiche() CreateSSNiche()
Create a SingleSampNiche object from analysis results
CreateSTIDObject()
Convert Seurat object to STID object
GeneEnrichment()
Perform Gene Enrichment Analysis (GO/KEGG or GSEA)
Gene_Geneset
Gene and Geneset File Structure
GetCoordInfo()
Get coordinate-related column names
GetGeneStat()
Calculate summary statistics for selected genes
GetInfo()
Get information from STID object
GetMetaData()
Get metadata from STID object
GetMetaInfo()
Get metadata information
GetMSNicheCells()
Get MultiSampNiche cells
GetMSNicheGenes()
Get MultiSampNiche genes
GetMSNicheInfo()
Get MultiSampNiche information
GetSapThreshold()
Retrieve sample-specific thresholds from previous analyses
GetSSNicheCells()
Get SingleSampNiche cells
GetSSNicheGenes()
Get SingleSampNiche genes
GetSSNicheInfo()
Get SingleSampNiche information
GetTopGenes()
Identify top expressed genes in the dataset
get_STID_globals()
Get STID global constants
get_STID_options()
Get STID options
GOID2Genelist()
Convert GO IDs to Gene Lists
h5ad2rds()
Convert AnnData h5ad file to Seurat rds file
MultiSampNiche-class MultiSampNiche
The MultiSampNiche Class
NicheDetect_Lasso()
Interactive lasso selection for ROI (Region of Interest) detection
NicheDetect_Spot()
Spot-level niche detection
NicheDetect_STS()
Automated ROI detection using density-based spatial clustering
NicheExpand()
Expand niche regions based on distance threshold
parse_gtf()
Parse GTF File into Tidy Data Frame
Plot_DistLine_Exp()
Plot Gene Expression Along Distance from Niche Center
Plot_DistLine_Ratio()
Plot Cell Type Ratio Along Distance from Niche Center
Plot_NicheCellComm()
Plot Niche Cell Communication Results
Plot_Spatial()
Create spatial plots for STID data
Plot_SpatialCoLoc()
Plot Spatial Co-localization
print(<STID>)
Print method for STID objects
rds2h5ad()
Convert Seurat rds file to AnnData h5ad file
RemoveMetaColumn()
Remove columns from existing metadata
RemoveMetaData()
Remove metadata from STID object
SetInfo()
Set information in STID object
Seurat_pipeline()
Standard Seurat Analysis Pipeline for Spatial Transcriptomics
SingleSampNiche-class SingleSampNiche
The SingleSampNiche Class
SpotDetect_Gene()
Detect infection spots based on individual gene expression
SpotDetect_Geneset()
Detect infection spots based on gene set scores
STID-class STID
The STID Class
STID_analysis-class STID_analysis
The STID_analysis Class
theme_common()
Common analysis theme
theme_spatial()
Spatial plot theme